Transgenic shRNA pigs reduce susceptibility to foot and mouth disease virus infection

نویسندگان

  • Shengwei Hu
  • Jun Qiao
  • Qiang Fu
  • Chuangfu Chen
  • Wei Ni
  • Sai Wujiafu
  • Shiwei Ma
  • Hui Zhang
  • Jingliang Sheng
  • Pengyan Wang
  • Dawei Wang
  • Jiong Huang
  • Lijuan Cao
  • Hongsheng Ouyang
  • Stephen P Goff
چکیده

Foot-and-mouth disease virus (FMDV) is an economically devastating viral disease leading to a substantial loss to the swine industry worldwide. A novel alternative strategy is to develop pigs that are genetically resistant to infection. Here, we produce transgenic (TG) pigs that constitutively expressed FMDV-specific short interfering RNA (siRNA) derived from small hairpin RNA (shRNA). In vitro challenge of TG fibroblasts showed the shRNA suppressed viral growth. TG and non-TG pigs were challenged by intramuscular injection with 100 LD50 of FMDV. High fever, severe clinical signs of foot-and-mouth disease and typical histopathological changes were observed in all of the non-TG pigs but in none of the high-siRNA pigs. Our results show that TG shRNA can provide a viable tool for producing animals with enhanced resistance to FMDV.

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عنوان ژورنال:

دوره 4  شماره 

صفحات  -

تاریخ انتشار 2015